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KMID : 0881720110260010016
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Journal of Food Hygiene and Safety
2011 Volume.26 No. 1 p.16 ~ p.24
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Simultaneous Determination of Eight Sugar Alcohols in Foodstuffs by High Performance Liquid Chromatography
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Lim Ho-Soo
Park Sung-Kwan
Kwak In-Shin
Kim Hyung-Il
Sung Jun-Hyun
Choi Jung-Yoon
Kim So-Hee
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Abstract
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A method was established for the simultaneous determinatioin of sugar alcohols, erythritol, xylitol, sorbitol, inositol, mannitol, maltitiol, lactitol and isomalt by High Performance Liquid Chromatography (HPLC). The sugar alcohols were converted into strong ultraviolet (UV)-absorbing derivatives with p-nitrobenzoyl chloride (PNBC). HPLC was performed on Imtakt Unison US-C18 column, using acetonitrile : water (77:23) as a mobile phase and UV detection (260 nm). The calibration curves for all sugar alcohols tested were linear in the 10~200 mg/L range. The average recoveries of the sugar alcohols from three confectioneries spiked at 100 ppm of eight sugar alcohol standards ranged from 81.2 to 123.1% with relative standard deviations ranging fromo 0.2 to 4.9%. The limits of detection (LODs) were 0.5~8 μg/L and the limits of quantification (LOQs) were 2~17 μg/L. Reproducibility of 8 sugar alcohols was 0.28~1.97 %RSD. The results of the analysis of confectioneries showed that 89 samples of 130 were detected and the sugar alcohols content of samples investigated varied between 0.4 and 693.7 g/kg. A method for the simultaneous determination of eight sugar alcohols will be used as basic data for control of sugar alcohols in confectioneries, and qualilty control in food manufacturing.
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KEYWORD
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Sugar alcohols, HPLC, p-Nitrobenzoyl chloride
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